Oxidized messenger RNA induces translation errors

Oxidized messenger RNA induces translation errors.

To investigate the effect of RNA oxidation on normal cellular functions, we studied the translation of nonoxidized and oxidized luciferase mRNA in both rabbit reticulocyte lysate and human HEK293 cells. When HEK293 cells transfected with nonoxidized mRNA encoding the firefly luciferase protein were cultured in the presence of paraquat, there was a paraquat concentration-dependent increase in th...

964 Transcription and Translation of / ~ Messenger Rna

Messenger R N A that code for the /x chain of secreted (s) and cell surface (m) immunoglobul in M (IgM) differ in size ~ m R N A are 2.4 kilobases [kb]; ~m m R N A are 2.7 kb) and in sequence at their 3' termini (1-4). These sequences predict two different carboxy terminal amino acid sequences and account for peptide (5-7) as well as hydrophobici ty (8, 9) differences that have been shown for t...

-methyladenosine Modulates Messenger RNA Translation Efficiency

Cell-free translation of paramyxovirus messenger RNA.

Polypeptides corresponding in electrophoretic mobility to virion polypeptides 1, 3, and 5 were made in a reticulocyte cell-free system to which 18S RNA from Sendai virus-infected cells was added. Immune precipitation was used to select relevant polypeptides from endogenous products. The cell-free product corresponding to virion polypeptide 3 (the nucleocapsid structure unit) was the most abunda...

N6-methyladenosine Modulates Messenger RNA Translation Efficiency

N(6)-methyladenosine (m(6)A) is the most abundant internal modification in mammalian mRNA. This modification is reversible and non-stoichiometric and adds another layer to the dynamic control of mRNA metabolism. The stability of m(6)A-modified mRNA is regulated by an m(6)A reader protein, human YTHDF2, which recognizes m(6)A and reduces the stability of target transcripts. Looking at additional...